This volume constitutes selected and revised papers presented during the Second International Conference on Trandisciplinary Multispectral Modelling and Cooperation for the Preservation of Cultural Heritage, TMM_CH 2021, held in Athens, Greece, in December 2021.The 17 full papers and 6 short papers presented in ths volume were thoroughly reviewed and selected from 310 submissions.The papers are organized in topical sections on ?scientific innovations in the diagnosis and preservation of cultural heritage; digital heritage a holistic approach; preservation, reuse and reveal of cultural heritage through sustainable land management, rural and urban development to recapture the world in crisis through culture.